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Technical Briefs

Immune Cell Biology

Available Assays Include Adoptive transfer of CD4+ helper T cell subsets derived from T cell receptor transgenic mice and subsequent immunization with cognate antigen. Adoptive transfer of CD8+ cytotoxic T cells derived from T cell receptor transgenic mice and subsequent analysis of antigen-specific cell killing. Injection of various TLR ligands (e.g., LPS, imiquimod) and analysis…

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LCMS/MS Bioanalytical Analysis

Sciex API 3000 HPLC/MS/MS Non-GLP Applications include Pharmacokinetic/Toxicokinetic analysis (PK/TK) Pharmacokinetic parameters calculated using WinNonlin Pharmacokinetic/Pharmacodynamic (PK/PD) Rapid discovery phase bioanalysis Tissue distribution Cassette dosing analysis In Vitro ADME Plasma/Serum/Matrix Stability Metabolic Stability (Liver microsomes, multiple species) Post translational protein modification Customized liquid chromatography – tandem mass spectrometry (HPLC/MS/MS) method development The early stages of…

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Luminex® Multiplex Assays

Applications include Signal transduction path way evaluation Sample Sources: Tissue culture conditioned media, whole blood / isolated PBMC, serum/plasma, biological fluids Species: human, mouse, rat, canine, non-human primate Analytes: Signaling proteins (phospho and total), Cytokines, Chemokines Data Generation: Concentration (cytokine, chemokine and signaling molecules), IC50determination As the information around signaling pathways and potentially drugable targets…

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MSD – Meso Scale Discovery

Applications include Signal transduction path way evaluation The Meso Scale Discovery (MSD®) platform provides a faster alternative to traditional ELISAs for performing sandwich immunoassays. The MSD® assay platform utilizes Ruthenium (II) tris-bipyridine-(4-methylsulfone) [Ru(bpy)3] that, once conjugated to the analyte, serves as the tracer in competitive assays. The Ru(bpy)3-based tag undergoes a rapid redox reaction that…

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Biacore® Surface Plasmon Resonance

Applications include Pharmaceutical drug discovery Antibody characterization Proteomics Immunogenicity Biotherapeutic development and manufacture Life science research applications Biacore® is a surface plasmon resonance (SPR) based analytical instrument. Biacore® technology allows generation of high quality data on the interactions between proteins and other molecules, including small molecule drug candidates. These data provide insights into molecular function…

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LI-COR® Odyssey CLX

Applications include In-Cell Western Assay Quantitative IR Western Blots Multiplex Detection The Odyssey® CLx Infrared Imaging System is a direct near-infrared fluorescence detection system. The imager is based upon the LI-COR industry-standard Odyssey Classic system. The Odyssey® CLx provides better data with its proven quantitative Western blot capability, while not compromising on the sensitivity researchers…

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Caliper Microfluidics

Applications include High thoughput screening Dermination of enzyme:inhibitor association and dissociation rate constants Microfluidic lab-on-a-chip technology incorporates the benefits of miniaturization, integration and automation to many research applications. Microfluidic chips are the key components of the LabChip® system, which also include experiment-specific reagents and software. The chips contain a network of miniaturized, microfabricated channels, containing…

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Fluorescent Activated Cell Sorting

Applications include: Immunophenotyping -4 color capability Biomarker analysis Cell viability (Annexin-PI) Intracellular staining Cell differentiation Absolute cell counts Calcium Flux Cell cycle and ploidy FACS is an essential tool for in-depth cell analysis. With the capacity to simultaneously measure multiple parameters on hundreds of individual cells per second, FACS is a powerful technology with a…

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