Posts Tagged ‘Cell Biology’
A Multiplexed Secreted Protein Assay Using Meso Scale Discovery® Technology
CASE STUDY: Custom Assay Development & Validation Synopsis A multiplex cytokine production assay using human peripheral blood mononuclear cells (PBMC) was developed and validated for use in a compound screening testing funnel as a functional cellular readout for ‘Target X’ modulation.
[Read More…]Fluorescent Activated Cell Sorting
Applications include: Immunophenotyping -4 color capability Biomarker analysis Cell viability (Annexin-PI) Intracellular staining Cell differentiation Absolute cell counts Calcium Flux Cell cycle and ploidy FACS is an essential tool for in-depth cell analysis. With the capacity to simultaneously measure multiple parameters on hundreds of individual cells per second, FACS is a powerful technology with a…
[Read More…]Multiplexed Analysis of Intracellular Phosphoproteins
CASE STUDY: Multiplex Phosphoprotein Analysis Synopsis A virtual translation research and development pharmaceutical company was seeking a cell-based biomarker for their drug development program. A multiplex phosphoprotein assay was developed to assess signaling pathway modulation in human cancer cells in response to treatment with selected compounds.
[Read More…]Multiplexed Analysis of Cytokine and Chemokine Profile in Endotoxin Challenged Mouse Bone Marrow Derived Macrophage Cells
CASE STUDY: Multiplexed Analysis of Cytokine and Chemokine Profile in Endotoxin Challenged Mouse Bone Marrow Derived Macrophage Cells Synopsis A multiplex murine cytokine/chemokine assay was used to assess the cytokine/chemokine profile in endotoxin challenged murine bone marrow derived macrophage cells.
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