Posts Tagged ‘Kinetics’
Biacore® Surface Plasmon Resonance
Applications include Pharmaceutical drug discovery Antibody characterization Proteomics Immunogenicity Biotherapeutic development and manufacture Life science research applications Biacore® is a surface plasmon resonance (SPR) based analytical instrument. Biacore® technology allows generation of high quality data on the interactions between proteins and other molecules, including small molecule drug candidates. These data provide insights into molecular function…
[Read More…]Understanding the Kinetics of Enzyme:Inhibitor Interactions
CASE STUDY: Understanding the Kinetics of Enzyme: Inhibitor Interactions Biochemistry & Enzymology Studies Synopsis The kinetics of human matrix metalloproteinase 2 (MMP-2) inhibition by galardin was determined.
[Read More…]Determination of p38α Kinase inhibitor dissociation rate constants using Microfluidic Technology
CASE STUDY: Determination of p38α Kinase inhibitor dissociation rate constants using Microfluidic Technology Synopsis A continuous kinase assay was employed using Caliper mobility shift technology to evaluate dissociation rates of a select group of p38 mitogen activated protein kinase (MAPKα) inhibitors and thereby differentiate inhibitory mechanisms.
[Read More…]Role of Enzyme:Inhibitor Kinetic Characterization in Predicting Pharmacodynamic Response of Irreversible Fatty Acid Amide Hydrolase (FAAH) Inhibitors
CASE STUDY: Role of Enzyme:Inhibitor Kinetic Characterization in Predicting Pharmacodynamic Response of Irreversible Fatty Acid Amide Hydrolase (FAAH) Inhibitors Synopsis To investigate the relevance of in vitro inhibitor kinetics to in vivo pharmacodynamics (PD), a simple fluorogenic assay was used to determine the kinetic constants of PF-3845, an irreversible FAAH inhibitor. The reported in vitro…
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