Case Studies
A Multiplexed Secreted Protein Assay Using Meso Scale Discovery® Technology
CASE STUDY: Custom Assay Development & Validation Synopsis A multiplex cytokine production assay using human peripheral blood mononuclear cells (PBMC) was developed and validated for use in a compound screening testing funnel as a functional cellular readout for ‘Target X’ modulation.
[Read More…]A Clinical Phosphoprotein Biomarker Assay
CASE STUDY: A Clinical Phosphoprotein Biomarker Assay Development, Validation, & Implementation Synopsis An assay was developed, validated and utilized in a clinical study to determine the activation of “Kinase 1” in human feces as a biomarker of colonic inflammation resulting from infection.
[Read More…]Multiplexed Analysis of Intracellular Phosphoproteins
CASE STUDY: Multiplex Phosphoprotein Analysis Synopsis A virtual translation research and development pharmaceutical company was seeking a cell-based biomarker for their drug development program. A multiplex phosphoprotein assay was developed to assess signaling pathway modulation in human cancer cells in response to treatment with selected compounds.
[Read More…]Understanding the Kinetics of Enzyme:Inhibitor Interactions
CASE STUDY: Understanding the Kinetics of Enzyme: Inhibitor Interactions Biochemistry & Enzymology Studies Synopsis The kinetics of human matrix metalloproteinase 2 (MMP-2) inhibition by galardin was determined.
[Read More…]Determination of p38α Kinase inhibitor dissociation rate constants using Microfluidic Technology
CASE STUDY: Determination of p38α Kinase inhibitor dissociation rate constants using Microfluidic Technology Synopsis A continuous kinase assay was employed using Caliper mobility shift technology to evaluate dissociation rates of a select group of p38 mitogen activated protein kinase (MAPKα) inhibitors and thereby differentiate inhibitory mechanisms.
[Read More…]Multiplexed Analysis of Cytokine and Chemokine Profile in Endotoxin Challenged Mouse Bone Marrow Derived Macrophage Cells
CASE STUDY: Multiplexed Analysis of Cytokine and Chemokine Profile in Endotoxin Challenged Mouse Bone Marrow Derived Macrophage Cells Synopsis A multiplex murine cytokine/chemokine assay was used to assess the cytokine/chemokine profile in endotoxin challenged murine bone marrow derived macrophage cells.
[Read More…]Role of Enzyme:Inhibitor Kinetic Characterization in Predicting Pharmacodynamic Response of Irreversible Fatty Acid Amide Hydrolase (FAAH) Inhibitors
CASE STUDY: Role of Enzyme:Inhibitor Kinetic Characterization in Predicting Pharmacodynamic Response of Irreversible Fatty Acid Amide Hydrolase (FAAH) Inhibitors Synopsis To investigate the relevance of in vitro inhibitor kinetics to in vivo pharmacodynamics (PD), a simple fluorogenic assay was used to determine the kinetic constants of PF-3845, an irreversible FAAH inhibitor. The reported in vitro…
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